Journal: International Journal of Molecular Medicine
Article Title: Potential regulatory role of circular RNA in idiopathic pulmonary fibrosis
doi: 10.3892/ijmm.2018.3892
Figure Lengend Snippet: Direct interaction between circRNA and miRNA. (A and B) Expression levels of miR-630 and miR-324-5p in IPF patients. ΔCt values were determined to quantify gene expression. Higher ΔCt value indicates lower expression. C. elegans miRNA 39-3p was used as a normalization control. Values are expressed as the mean ± standard deviation (n=10). ** P<0.01 vs. the NC group measured via unpaired Student’s t-tests. (C) Wild-type and mutated miR-324-5p binding site on hsa_circRNA_100906. (D) hsa_circRNA_102348 sequences containing wild-type or mutated miR-630 binding sites. (E and F) Dual-luciferase reporter assay demonstrated direct binding or miR-324-5p and miR-630 with circRNA-100906 and circRNA-102348, respectively. Values are expressed as the mean ± standard deviation. *** P<0.001; ### P<0.001 measured via analysis of variance and Student-Newman-Keuls test. miR/miRNA, microRNA; IPF, idiopathic pulmonary fibrosis; circRNA, circular RNA; NC, normal control; WT, wild-type; MT, mutated type; Luc, luciferase; R, Renilla .
Article Snippet: The miRNA response elements on circRNA and mRNA were scanned using miRNA target prediction software (version 1.0; Arraystar Inc.) based on miRanda and Target Scan.
Techniques: Expressing, Standard Deviation, Binding Assay, Luciferase, Reporter Assay